|
KMID : 0380019940090010001
|
|
Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 1 p.1 ~ p.7
|
|
|
Mechanism of Dextrans Synthesis by Dextransucrase
|
|
|
|
|
Abstract
|
|
|
|
A qualitative study was made on the mechanism of dextran synthesis by dextransucrase. Enzymatic synthesis of dextran was experimentally studied with initial sucrose concentration from 50 g/P to 150 g/P. The molecular weight distribution of synthesized dextran was measured by using on-line gel permeation chromatographic system. Sucrose was observed not to work as a primer within the range of concentration tested. At the initial sucrose concentration of 50 g/P, dextran with molecular weight of medium range (10´-2x10´) was synthesized due to the mass transfer limitation of sucrose. The amount of the dextran of medium range decreased with the initial sucrose concentration. Dextran was likely to be synthesized by radical chain polymerization mechanism since the dextran of medium range was not produced at higher sucrose concentrations.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|